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Aberrant regulation of Wnt/β-catenin signaling is a standard concept noticed across quite a few human cancer types 52, 53. According to this concept, our results confirmed that the expression of genes linked to the Wnt/β-catenin pathway, for example TCF4At 32 hpf, Wnt/β-catenin signaling is active in non-fully commited, proliferative progenitors